Commercial Crystallization Kits

The Crystallization Facility utilizes commercial kits from Emerald Biosystems, Hampton Research, and Qiagen. For initial screening of samples we typically recommend 4-8 screens consisting of: Crystal Screen I-II, Index, JCSG+, PACT, PEG-ion/Cryo, SaltRx, Wizard I-II, and AmSO₄.

Please note, only underlined screens are kept ready to use for crystallization trials. Please notify us in advance if you would like to perform less common screens. They are typically ready within 24 hours.

If you're curious about the amount of overlap between the screens below, download this (excel):
Crystallization Screen Comparison

Download Composition Tables & Scoring Sheets

Molecular Dimensions
www.moleculardimensions.com

Structure Screen (SS)

sparse matrix, broad screen of salts, polymers, organics, and pH

Composition ( 1-48, 49-96 )

MemGold (MemGold)

sparse matrix, designed for alpha and beta membrane proteins
Composition (pdf)

MIDAS (MIDAS)

sparse matrix, unconventional and synthetic organics

Composition (pdf)

Emerald Biosystems
www.emeraldbiosystems.com

    Emerald Additive Screen Optimization
        96 unique additives
        Composition (pdf)
    Wizard
        sparse matrix, broad screen of buffers, crystallants, and pH
        Composition (pdf), Scoring Sheet (pdf)

Hampton Research
www.hamptonresearch.com

    Additive Screen Optimization
        96 unique additives
        Composition (pdf), Scoring Sheet (pdf)
    Detergent Screen Optimization
        96 unique detergents, ideal for membrane proteins
        Composition (pdf), Scoring Sheet (pdf)
    Index (ind)
        grid, sprase matrix, broad screen of salts, pH, PEG, and organics
        Composition (pdf), Scoring Sheet (pdf)
    PEG
        sparse matrix
        Composition (pdf, pdf), Composition (Excel , Excel) Scoring Sheet (pdf, pdf)
    SaltRx
        sparse matrix, salt & pH only
        Composition (pdf , Excel), Scoring Sheet (pdf)
    Silver Bullets Optimization
        96 solutions using small molecule/macromolecular digests
        Composition (pdf), Scoring Sheet (pdf)

Qiagen
www.qiagen.com

    AmSO4
        grid/sparse matrix/ionic
        Composition (pdf , Excel), Scoring Sheet (pdf)
    ComPAS
        sparse matrix, salts/organics/polymers as precipitants
        Composition (pdf , Excel), Scoring Sheet (pdf)

    Cryos
        sparse matrix, glycerol as cryoprotectant
        Composition (pdf , Excel), Scoring Sheet (pdf)

    JCSG+
        sparse matrix, mostly polymers as preciptitant
        Composition (pdf , Excel), Scoring Sheet (pdf)

    MbClass
        sparse matrix, designed for membrane proteins
        Composition (pdf , Excel), Scoring Sheet (pdf)

    MbClass II
        sparse matrix, designed using conditions of alpha-helical proteins
        Composition (pdf , Excel), Scoring Sheet (pdf)

    MPDs
        grid/sparse matrix/ionic, 2-methyl-2,4-pentandiol organic precipitant
        Composition (pdf , Excel), Scoring Sheet (pdf)

    Nucleix
        sparse matrix, designed for nucleic acid/protein-nucleic acid complexes
        Composition (pdf , Excel), Scoring Sheet (pdf)

    PACT
        grid, pH/ionic screen with PEG as precipitant
        Composition (pdf , Excel), Scoring Sheet (pdf)

    ProComplex
        sparse matrix, designed for protein-protein complexes
        Composition (pdf , Excel), Scoring Sheet (pdf)