Adapted from Dauter et al., 2000, Novel approach to phasing proteins: derivatization by short cryo-soaking with halides. Acta Cryst vol D56, 232-237.:

(1) KI pellets are located in room 219A, in the chemical cabinet with sliding glass doors. In small white plastic bottle.
(2) Weigh 0.008g KI (one medium sized grain)
(3) Dissolve KI in 100 uL of reservoir solution. (Makes 0.5M KI solution) (KI=166 g/mol)
(4) Mix 6.5 microliters KI solution with 3.5 microliters 100% glycerol stock for cryoprotection. (more or less glycerol may be required for cryoprotection)
(5) Soak crystal 30 seconds in the cryo-KI solution.
(6) Mount crystal on cold stream

For additional information on Mike's iodide experiences at UCLA see Phasing with potassium iodide- a powerpoint presentation.

Adapted from Beck et al., 2008, A magic triangle for experimental phasing of macromolecules. Acta Cryst vol D64, 1179-1182. and Beck et al., 2009, How to get the magic triangle and the MAD triangle into your protein crystal. Acta Cryst vol F65, 1068-70.

I3C has the advantage of 3 iodide atoms per molecule for additional phasing power. See Tobias Beck's web page for additional information.

(1) I3C is located in room 219A, in the drawer below the balance. The drawer is labeled "Heavy atom ends". In a small brown glass bottle with red lid. It was ordered from Sigma http://www.sigmaaldrich.com/catalog/search/ProductDetail/ALDRICH/444367
(2) Weigh 0.03g I3C (30 mg)
(3) Dissolve I3C in 59 uL of 2.0 M lithium hydroxide. (Makes 1.0M I3C solution) (I3C=556 g/mol) as recommended by Tobias.
(4) Mix 3.5 microliters I3C solution with 6.5 microliters reservoir and 3.5 microliters 100% glycerol stock for cryoprotection. This makes approximately 260 mM I3C, final concentration. Tobias recommends up to 500mM concentration. (more or less glycerol may be required for cryoprotection)
(5) Soak crystal 5 minutes in the cryo-I3C solution.
(6) Mount crystal on cold stream

As a positive control, I3C was used to derivatize proteinase K. You can download the XDS formatted structure factors or the Scalepack formatted structure factors and log file.

Adapted from Miyatake H, Hasegawa T, Yamano A. New methods to prepare iodinated derivatives by vaporizing iodine labelling (VIL) and hydrogen peroxide VIL (HYPER-VIL). Acta Crystallogr D Biol Crystallogr. 2006 Mar;62(Pt 3):280-9.:

(1) Find KI (potassium iodide) in room 269A, in the wooden cabinet near the shaker room, in a small white plastic bottle. Find I2 (elemental iodine) in the same room, in the blue cabinet near the hood, in a bin labeled "toxic and carcinogen", in a medium brown jar.
(2) Weigh 0.250 g KI in Eppendorf tube. (KI=166 g/mol)
(3) Dissolve KI in 1 mL of water.
(4) Weigh 0.135 g I2 in another Eppendorf tube. (I2 = 126.9 g/mol)
(5) Transfer the KI solution to the solid I2 to dissolve. (According to the paper, this makes 0.67 M KI/ 0.47 M I2, but by my calculations, this is 1.5 M KI/ 1.06 M I2 )
(6) Store at -20 deg C in 50 microliter aliquots
(7) Remove glass coverslip containing the drop with the crystal to be derivatized. Place 0.2 microliters on coverslip next to crystal drop, NOT touching the drop.
(8) Invert the coverslip over the reservoir as it was before you removed it.
(9) Incubate for 30 minutes to 24 hours. VIL derivative is ready!
(10) If you need to increase reactivity, add 1 microliter of 30% hydrogen peroxide on the coverslip, separate from the drops containing KI/I2 and the crystal. This is HYPER-VIL.

Adapted from Nagem RA, Dauter Z, Polikarpov I. Protein crystal structure solution by fast incorporation of negatively and positively charged anomalous scatterers. Acta Crystallogr D Biol Crystallogr. 2001 Jul;57(Pt 7):996-1002.:

(1) CsCl is located in room 269A, in the wooden cabinet near the shaker room. It is in a clear plastic 1L bottle.
(2) Weigh 0.017g CsCl in Eppendorf tube.
(3) Dissolve CsCl in 100 uL of reservoir solution. (Makes 1.0M CsCl solution) (CsCl=168.36 g/mol)
(4) Mix 6.5 microliters CsCl solution with 3.5 microliters 100% glycerol stock for cryoprotection if necessary. (more or less glycerol may be required for cryoprotection)
(5) Soak crystal 60 seconds in the cryo-CsCl solution.
(6) Mount crystal on cold stream