TUTORIAL #2B: Sequence Alignment 1-D to 1-D

This Tutorial will teach you how to obtain:

the name of the gene used in the exercise

the full nucleic acid sequence

the full protein sequence

a list of enzymes that do not cut the nucleic acid sequence

Perform a blast search at BCM using the following sequence:
CCCGGGGATG GCTCCGTGGC CTCACGAGAA CAGCTCTCTT GCCCCATGGC CGGACCTCCC
(Highlight the sequence, then choose copy from the Edit menu. Go to the Blast search page and Edit, Paste the sequence into the sequence box)

To fetch the complete DNA sequence of the gene, click on the E icon (Entrez) next to the first aligned sequence. Then on the top page click on DNA links, then on GenBank report.

Go to the bottom of that page, copy the gene sequence (and only the gene sequence, no words or other stuff,numbers on the left are OK) to the clipboard (Edit, copy - just like before).

Go to the Sequence Utility of the BCM launcher and perform a Webcutter restriction enzyme search. (Edit, Paste the sequence into the sequence box) At the bottom of the Webcutter results you will find a list of restriction enzymes that WILL NOT cut the sequence.

Go back to the Sequence Utilities page (click back) and perform a six reading frame translation using the same sequence.

Copy the most likely protein translation (in this case the correct reading frame is the one with ONLY ONE asterisk -*- ) to the clipboard.