DNA Duplex, PDB#111D

1) Add 0.1 volume of either 3M NaOAc or 10M NH4OAc to the DNA sample

2) Add 2 volumes of EtOH to the mixture to precipitate te DNA

3) Place the sample on ice for about 20 min.

4) Centrifuge at max speed for 20 min.

5) Pipet out the supernatant and discard it (Note: be careful not to disturb the DNA pellet)

6) Wash the DNA pellet with 1mL 70% EtOH

7) Air-dry the DNA pellet in a desiccator or a 37oC incubator for about 10 min.

8) Add whatever desired volume of H2O or TE buffer to dissolve the DNA pellet

This process usually gives about an 80% yield


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