Note : The compositions of the buffers listed below are those of 1X concentration

REact 2 Buffer
50mM Tris-HCl, pH8.0
10mM MgCl₂
50mM NaCl

REact 3 Buffer
50mM Tris-HCl, pH8.0
10mM MgCl₂
100mM NaCl

REact 6 Buffer
50mM Tris-HCl, pH7.4
6mM MgCl₂
50mM NaCl
50mM KCl

NcoI
10mM Tris-HCl (pH 7.5)
50mM KCl
0.1mM EDTA
1mM DTT
100mM ug/mL BSA
50% (v/v) glycerol
0.1% (w/v) TRITON X-100

NotI
20mM Tris-HCl (pH 7.5)
100mM KCl
0.1mM EDTA
1mM DTT
500mM ug/mL BSA
50% (v/v) glycerol
0.01% (w/v) TRITON X-100

PstI
10mM Tris-HCl (pH 7.5)
50mM NaCl
0.1mM EDTA
1mM DTT
50% (v/v) glycerol
0.15% (w/v) TRITON X-100

Suggested Procedure of Sequential Digest using NdeI & NotI
1) First digest with NdeI in REact 2 Buffer for 3 hours, or more if you feel like it
2) Then digest with NotI under condition of REact 3 Buffer
3) Add extra NaCl to the reaction and bring it up to 100mM final conc. to create this condition for NotI
4) Incubate the reaction at 37^oC for 3 hours or more

Note: Unit definition - One unit is defined as the amount of enzyme required to completely digest 1ug of DNA in one hour at 37^oC

Here's a website with more info of Gibco enzyme buffers and their compositions

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