Once you find a condition that produces crystals, you may want to consider an optimization. Optimization trays expand a single condition over the X andf Y-axes of a plate, varying 2 of the components. Performing optimizations on previously successful conditions can lead to the discovery of "optimal conditions" for the crystallization of your sample, and larger crystals.

The Crystallization Facility is able to design customized screens based on hits from initial screening with commercial kits. We utilize OptiCrys to design the optimization plate and generate an excel file of liquid values, which is imported into our Tecan Genesis pipetting robot.

Options for creating your optimization

The facility sets up the optimization for you, and creates the plate
This process involves some discussion of the desired optimization parameters, and all pipetting values are setup using OptiCrys in the facility. We then use the facility's robot to pipett the liquids for you, and then setup the crystallization experiment.

You set up your own optimization, and use the facility to create the plate
Alternatively, you may setup the optimization parameters, create an excel file with all pipetting values, and simply use the facility's robot to pipett the liquids for you. We then setup the crystallization experiment using the optimization plate.

If considering this route, please be aware of the following:
    - volumes of each reagent must be greater than 5 ul in all wells
    - the robot is unable to pipett high % PEG stock solutions
    - OptiCrys does not correctly set up pH gradients


Please format your excel file like the following:

Well	Salt	Precipitant	Buffer	Water
A1	15	20	25	40
A2	17	20	25	38
A3	19	20	25	36
A4	21	20	25	34
A5	23	20	25	32

 
In addition, you may want to use this tool from Emeral Biosystems that enables the user to select a condition to optimize, and provides suggested ranges. Screen manufacturers may be identified under the "Screens Available" link above.

http://www.emeraldbiosystems.com/escreentoolkit/escreen.html

What we need to make your optimization

1. A detailed description of what the optimization conditions should be.
2. Any chemical reagents to be used in the optimization plate (including water).
3. Signed billing form.
4. At least 20ul of sample (this translates to 200nl of sample/drop).
5. Special instructions about your sample.
6. In addition, if you intend to vary buffer pH, do so along the Y-axis of your plate, and provide 8 pre-made 15 ml sample tubes to dispense along the x-axis.